This study investigates the role of applying the individual signature strengths at work for positive experiences at work (i.e. job satisfaction pleasure engagement and meaning) and calling. A sample of 111 employees from various occupations completed measures on character strengths positive experiences at work and calling. Coworkers (N¼111) rated the applicability of character strengths at work. Correlations between the applicability of character strengths and positive experiences at work decreased with intraindividual centrality of strengths (ranked strengths from the highest to the lowest). The level of positive experiences and calling were higher when four to seven signature strengths were applied at work compared to less than four. Positive experiences partially mediated the effect of the number of applied signature strengths on calling. Implications for further research and practice will be discussed.